Brackets:
| Region/Match | Matchup | Date/Time/Result |
|---|---|---|
| Atlantic 1 | ||
| 1 | Shaw (13-4) vs. Chowan (15-8) | Shaw, 5-2 |
| 2 | *Bluefield State (17-3) vs. Shaw (14-4) | Bluefield State, 5-0 |
| Atlantic 2 | ||
| 1 | *Charleston (W.Va.) (13-3) vs. Edinboro (13-6) | Edinboro, 5-4 |
| 2 | Kutztown (11-3) vs. West Virginia Wesleyan (13-3) | Kutztown, 5-2 |
| 3 | Edinboro (14-6) vs. Kutztown (12-3) | Edinboro, 5-3 |
| Central 1 | ||
| 1 | No matches | Northwest Mo. St. (16-2) |
| Central 2 | ||
| 1 | *Southwest Baptist (10-9) vs. Nebraska-Kearney (12-5) | Southwestern Baptist, 5-3 |
| East 1 | ||
| 1 | Southern New Hampshire (13-3) vs. Merrimack (12-7) | Merrimack, 5-3 |
| 2 | *Concordia (N.Y.) (15-2) vs. Merrimack (13-7) | Concordia (N.Y.), 5-0 |
| East 2 | ||
| 1 | *NYIT (15-4) vs. Queens (N.Y.) (9-7) | NYIT, 5-0 |
| 2 | Adelphi (11-5) vs. Chestnut Hill (18-5) | Chestnut Hill, 5-1 |
| 3 | NYIT (16-4) vs. Chestnut Hill (19-5) | NYIT, 5-1 |
| Midwest 1 | ||
| 1 | *Drury (19-1) vs. Grand Valley State (11-5) | Drury, 5-0 |
| 2 | Wayne State (Mich.) (10-7) vs. Southern Indiana (15-6) | Southern Indiana, 5-3 |
| 3 | Drury (20-1) vs. Southern Indiana (16-6) | Drury, 5-2 |
| Midwest 2 | ||
| 1 | *Indianapolis (12-2) vs. Missouri-St. Louis (10-5) | Indianapolis, 5-3 |
| 2 | McKendree (7-5) vs. Northwood (Mich.) (15-3) | Northwood (Mich.), 5-3 |
| 3 | Indianapolis (13-2) vs. Northwood (Mich.) (16-3) | Northwood (Mich.), 5-3 |
| South 1 | ||
| 1 | West Alabama (20-2) vs. Valdosta State (12-7) | Valdosta State, 5-2 |
| 2 | *West Florida (20-0) vs. Valdosta State (13-7) | West Florida, 5-0 |
| South 2 | ||
| 1 | *Barry (17-2) vs. Stillman (16-3) | Barry, 5-0 |
| 2 | Rollins (12-8) vs. Lynn (11-6) | Lynn, 5-1 |
| 3 | Barry (18-2) vs. Lynn (12-6) | May 6, 11 a.m. ET |
| South Central 1 | ||
| 1 | St. Edward's (12-2) vs. Colorado Mesa (9-5) | St. Edward's, 5-1 |
| 2 | *Midwestern State (14-4) vs. St. Edward's | Midwestern State, 5-4 |
| South Central 2 | ||
| 1 | *Cameron (12-4) vs. Western New Mexico (11-5) | Cameron, 5-2 |
| Southeast 1 | ||
| 1 | North Georgia (10-9) vs. South Carolina-Aiken (10-9) | South Carolina-Aiken, 5-2 |
| 2 | Georgia College (14-7) vs. GRU Augusta (16-6) | GRU Augusta, 5-2 |
| 3 | *Armstrong Atlantic (16-5) vs. South Carolina-Aiken (11-9) | Armstrong Atlantic, 5-0 |
| 4 | GRU Augusta (17-6) vs. Armstrong Atlantic (17-5) | Armstrong-Atlantic, 5-0 |
| Southeast 2 | ||
| 1 | *Columbus State (17-4) vs. Wingate (16-4) | Columbus State, 5-0 |
| 2 | Mount Olive (18-0) vs. Lander (16-3) | Lander, 5-0 |
| 3 | Columbus State (18-4) vs. Lander (17-3) | Columbus State, 5-4 |
| West 1 | ||
| No matches | Hawaii Pacific (17-0) | |
| West 2 | ||
| No matches | UC San Diego (11-6) | |
| *Host school | ||
Note: The winner of the last match listed in each region advances to the site of the final 16 in Altamonte Springs, Fla..
