Brackets:
| REGION / MATCH | MATCHUP | DATE / RESULT |
|---|---|---|
| Atlantic 1 | ||
| 1 | Mercyhurst (10-4) vs. West Liberty (13-8) | Mercyhurst, 5-4 |
| 2 | *California (Pa.) (12-1) vs. Mercyhurst (11-4) | California (Pa.), 5-0 |
| Atlantic 2 | ||
| 1 | *Indiana (Pa.) (15-6) vs. Chowan (21-5) | Indiana (Pa.), 5-0 |
| 2 | Slippery Rock (11-9) vs. West Virginia State (13-2) | West Virginia State, 5-1 |
| 3 | Indiana (Pa.) (16-6) vs. West Virginia State (14-2) | Indiana (Pa.), 5-3 |
| Central 1 | ||
| 1 | Harding (15-4) vs. Arkansas Tech (11-4) | Arkansas Tech, 5-4 |
| 2 | *Southwest Baptist (21-2) vs. Arkansas Tech (12-4) | Southwest Baptist, 5-1 |
| Central 2 | ||
| 1 | *Augustana (S.D.) (19-0) vs. Emporia State (13-8) | Augustana (S.D.), 5-0 |
| 2 | Nebraska-Kearney (14-7) vs. Northeastern State (17-4) | Northeastern State, 5-1 |
| 3 | Augustana (S.D.) (20-0) vs. Northeastern State (18-4) | Northeastern State, 5-3 |
| East 1 | ||
| 1 | Chestnut Hill (18-7) vs. Merrimack (14-3) | Chestnut Hill, 5-0 |
| 2 | *NYIT (20-4) vs. Chestnut Hill (19-7) | NYIT, 5-0 |
| East 2 | ||
| 1 | *Queens (N.Y.) (16-4) vs. Goldey-Beacom (11-5) | Queens (N.Y.), 5-0 |
| 2 | Concordia (N.Y.) (11-5) vs. Queens (N.Y.) (17-4) | Concordia (N.Y.), 5-2 |
| Midwest 1 | ||
| 1 | Lewis (13-3) vs. Grand Valley State (14-7) | Lewis, 5-0 |
| 2 | *Drury (18-4) vs. Lewis (14-3) | Lewis, 5-0 |
| Midwest 2 | ||
| 1 | *Northwood (Mich.) (20-0) vs. Tiffin (18-6) | Northwood, 5-3 |
| 2 | Missouri-St. Louis (15-5) vs. Wayne State (Mich.) (21-4) | Wayne State, 5-2 |
| 3 | Wayne State (Mich.) (22-4) vs. Northwood (Mich.) (21-0) | Wayne State, 5-3 |
| South 1 | ||
| 1 | West Florida (23-4) vs. Rollins (15-5) | West Forida, 5-2 |
| 2 | *Barry (18-0) vs. West Florida (24-4) | Barry, 5-1 |
| South 2 | ||
| 1 | Lynn (15-3) vs. Stillman (22-2) | Lynn, 5-0 |
| 2 | *Saint Leo (17-5) vs. Lynn (16-3) | Saint Leo, 5-3 |
| South Central 1 | ||
| 1 | *Midwestern State (18-3) vs. Colorado Mesa (14-3) | Midwestern State, 5-0 |
| South Central 2 | ||
| 1 | *Saint Mary's (Texas) (11-4) vs. Cameron (15-5) | Saint Mary's (Texas), 5-0 |
| Southeast 1 | ||
| 1 | *Armstrong Atlantic (22-1) vs. Tusculum (19-2) | Armstrong Atlantic, 5-0 |
| 2 | Wingate (17-2) vs. Francis Marion (14-6) | Francis Marion, 5-3 |
| 3 | Armstrong Atlantic (23-1) vs. Francis Marion (15-6) | Armstrong Atlantic, 5-0 |
| Southeast 2 | ||
| 1 | *Columbus State (19-3) vs. Erskine (18-4) | Columbus State, 5-0 |
| 2 | Georgia College (17-7) vs. Flagler (16-4) | Georgia College, 5-1 |
| 3 | Columbus State (20-3) vs. Georgia College (18-7) | Columbus State, 5-0 |
| West 1 | ||
| No matches | BYU-Hawaii (26-0) | |
| West 2 | ||
| *Hawaii Pacific (13-5) vs. Academy of Art (16-3) | Hawaii Pacific, 5-0 | |
| * -- Host school | ||
| The winner of the last match listed in each region advances to the site of the final 16 in Altamonte Springs, Fla. | ||